Thin Layer Chromatography Worksheet
A Grade 10 science worksheet covering the principles and applications of Thin Layer Chromatography (TLC).
Includes
Standards
Topics
Thin Layer Chromatography
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Read each question carefully and answer to the best of your ability. Show all work where applicable.
1. Which of the following best describes the stationary phase in Thin Layer Chromatography (TLC)?
The solvent that moves up the plate
The sample being separated
A thin layer of adsorbent material on a plate
The chamber where separation occurs
2. What is the primary principle behind the separation of components in TLC?
Differences in boiling points
Differences in solubility and adsorption to the stationary phase
Differences in magnetic properties
Differences in electrical conductivity
1. In TLC, the ratio of the distance traveled by the solute to the distance traveled by the solvent front is known as the value.
2. The process where the mobile phase moves up the stationary phase by capillary action is called .
3. A common adsorbent material used as the stationary phase in TLC is .
1. Briefly explain the roles of the mobile phase and the stationary phase in achieving separation in Thin Layer Chromatography.
2. Describe two factors that can affect the Rf value of a compound in TLC.
1. A compound with a higher affinity for the mobile phase will travel a shorter distance on the TLC plate.
True
False
2. Visualization agents are only needed for colored compounds in TLC.
True
False
Match each term with its correct definition.
1. Mobile Phase
a. The distance traveled by the compound divided by the distance traveled by the solvent front.
2. Stationary Phase
b. The solvent (or mixture of solvents) that moves through the stationary phase.
3. Rf Value
c. The adsorbent material (e.g., silica gel) coated on a plate.
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