Plasmid Mapping Worksheet
This worksheet covers key concepts in plasmid mapping, including restriction enzymes, recognition sites, and constructing a plasmid map.
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Plasmid Mapping Worksheet
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Read each question carefully and provide concise answers. Use the provided space for your responses.
1. Which of the following is NOT a characteristic of a restriction enzyme recognition site?
Usually 4-8 base pairs long
Palindromic sequence
Randomly distributed throughout the genome
Often produces sticky ends
2. What is the primary purpose of plasmid mapping?
To determine the exact DNA sequence of a plasmid
To identify the location of specific genes on a plasmid
To construct recombinant DNA molecules
To analyze protein expression from a plasmid
3. Restriction enzymes cut DNA at specific sites.
4. The process of separating DNA fragments by size is called .
5. A plasmid map is a circular diagram that shows the positions of restriction enzyme sites and other important on a plasmid.
6. Explain the difference between 'sticky ends' and 'blunt ends' produced by restriction enzymes and their significance in molecular cloning.
7. Describe the basic steps involved in creating a restriction map of a plasmid using single and double digestions.
8. All restriction enzymes cut DNA at the same recognition sequence.
True
False
9. The size of DNA fragments can be estimated by comparing them to a DNA ladder during gel electrophoresis.
True
False
10. Consider a circular plasmid of 5000 base pairs (bp). Restriction enzyme A cuts at 1000 bp and 3000 bp. Restriction enzyme B cuts at 2000 bp and 4500 bp. Draw a linear restriction map and a circular plasmid map showing the cut sites and resulting fragment sizes for single digests with A, B, and a double digest with A+B.
Linear Map:
Circular Map: