Thin Layer Chromatography (TLC) Worksheet
Grade 11 Chemistry worksheet on Thin Layer Chromatography covering principles, applications, and calculations.
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Thin Layer Chromatography (TLC) Worksheet
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Read each question carefully and provide your answers in the space provided. For multiple-choice questions, circle the correct option. For calculations, show all your work.
Thin Layer Chromatography (TLC) is a widely used analytical technique for separating non-volatile mixtures. It is a quick, inexpensive, and simple method to monitor the progress of reactions, identify compounds present in a given substance, and determine the purity of a substance.
1. Which of the following is the stationary phase in Thin Layer Chromatography?
Solvent
Mobile phase
Adsorbent coated plate
Analyte
2. What is the primary principle behind the separation of compounds in TLC?
Boiling point differences
Differences in solubility and adsorption
Molecular weight differences
Density differences
3. Briefly explain the role of the mobile phase in TLC.
4. List two common applications of Thin Layer Chromatography in a chemistry laboratory.
5. The distance traveled by a compound relative to the solvent front is known as the factor.
6. A TLC plate is typically made of a solid support (like glass, plastic, or aluminum foil) coated with a thin layer of material.
Refer to the diagram below showing a typical Thin Layer Chromatography setup.

7. In a TLC experiment, compound X traveled 4.5 cm from the origin, and the solvent front traveled 9.0 cm. Calculate the Rf value for compound X. Show your work.
8. A higher Rf value indicates that the compound has a stronger interaction with the stationary phase.
True
False