Grade 12 Biotechnology Worksheet
This worksheet covers fundamental concepts in biotechnology for Grade 12 students, including DNA technology, genetic engineering, and applications.
Includes
Standards
Topics
Biotechnology Fundamentals
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Read each question carefully and answer to the best of your ability. Show all your work for calculations.
1. Which of the following enzymes is used to cut DNA at specific recognition sequences?
DNA Polymerase
RNA Ligase
Restriction Enzyme
Helicase
2. What is the primary purpose of PCR (Polymerase Chain Reaction) in biotechnology?
To sequence DNA
To amplify specific DNA segments
To cut DNA into fragments
To separate DNA fragments by size
3. The process of inserting foreign DNA into an organism's genome is called .
4. Plasmids are commonly used as in genetic engineering to transfer genes between organisms.
5. CRISPR-Cas9 is a revolutionary gene-editing tool that allows for precise modification of sequences.
6. Briefly explain the concept of recombinant DNA technology.
7. Describe one ethical concern associated with genetic engineering in humans.
8. Gel electrophoresis separates DNA fragments based on their charge and size.
True
False
9. Transgenic organisms are those that contain genes from another species.
True
False
10. Observe the diagram of DNA below and answer the following questions.
a) What are the main components that make up a nucleotide?
b) How do the two strands of DNA remain connected in the double helix structure?