Thin Layer Chromatography (TLC) Worksheet
Grade 12 Chemistry worksheet covering the principles, applications, and interpretation of Thin Layer Chromatography.
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Thin Layer Chromatography (TLC) Worksheet
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Read each question carefully and provide concise answers. Show all calculations where necessary.
1. Which of the following best describes the stationary phase in Thin Layer Chromatography?
A liquid solvent that moves through the plate.
A solid adsorbent coated on a plate.
The sample being separated.
The chamber where chromatography takes place.
2. What property of a compound primarily determines its Rf value in TLC?
Its molecular weight.
Its melting point.
Its polarity and solubility in the mobile phase.
Its color.
3. In TLC, the separation of components is based on their differential partitioning between the phase and the phase.
4. A compound with a higher affinity for the stationary phase will have a Rf value.
5. Visualizing spots on a TLC plate can be done using UV light or by applying a agent.
6. Describe the general procedure for performing a Thin Layer Chromatography experiment.
7. Explain the term 'Rf value' and how it is calculated in TLC. What information does it provide about a compound?
Examine the image below, which depicts a typical Thin Layer Chromatography setup.
8. Based on the image and your knowledge, label the following components:
a) Stationary phase:
b) Mobile phase:
c) Spotting line/Origin:
d) Solvent front:
9. A more polar solvent will generally result in higher Rf values for most compounds on a silica gel TLC plate.
True
False
10. TLC can be used to determine the purity of a sample.
True
False