Grade 9 Plasmid Mapping Worksheet
This worksheet focuses on understanding plasmid structure, restriction enzymes, and gel electrophoresis in the context of plasmid mapping for Grade 9 science students.
Includes
Standards
Plasmid Mapping: Grade 9 Science
Name:
Date:
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Read each question carefully and answer to the best of your ability. Show all your work for calculations.
1. A plasmid is a small, circular piece of found in bacteria.
2. enzymes are proteins that cut DNA at specific recognition sequences.
3. The process of separating DNA fragments by size using an electric current is called .
1. Which of the following is NOT a typical feature of a plasmid?
Origin of replication
Centromere
Antibiotic resistance gene
Multiple cloning site
2. If a circular plasmid is cut once by a restriction enzyme, how many DNA fragments will be produced?
One
Two
Three
Depends on the plasmid size
1. Restriction enzymes always cut DNA at random locations.
True
False
2. Smaller DNA fragments travel faster through an agarose gel during electrophoresis.
True
False
1. Briefly explain the purpose of a multiple cloning site (MCS) in a plasmid.
2. Describe how gel electrophoresis helps in mapping a plasmid.
Observe the hypothetical plasmid diagram below and answer the questions.
1. If the plasmid shown above is cut with EcoRI only, how many linear DNA fragments would you expect to see on a gel?
2. If the plasmid is cut with both EcoRI and BamHI, how many fragments would be produced, and why?
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