Thin Layer Chromatography Worksheet
Explore the principles and applications of Thin Layer Chromatography (TLC) with this worksheet designed for grade 9 science students.
Includes
Standards
Topics
Thin Layer Chromatography
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Date:
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Read each question carefully and answer to the best of your ability. Use complete sentences for short answer questions.
1. What is the primary purpose of Thin Layer Chromatography (TLC)?
To synthesize new compounds
To separate components of a mixture
To measure the boiling point of a substance
To determine the mass of a sample
2. In TLC, the stationary phase is typically a thin layer of adsorbent material coated on a plate. Which of the following is commonly used as an adsorbent?
Water
Ethanol
Silica gel
Cooking oil
3. In TLC, the mobile phase moves up the stationary phase by capillary action.
True
False
4. A higher Rf value indicates that a compound has a stronger interaction with the stationary phase.
True
False
5. The ratio of the distance traveled by the solute to the distance traveled by the solvent front is known as the value.
6. In TLC, a substance that is more soluble in the mobile phase and less adsorbed by the stationary phase will travel up the plate.

7. Briefly explain the roles of the stationary phase and the mobile phase in Thin Layer Chromatography.
8. What factors influence the separation of compounds in TLC?
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