Monohybrid Cross Worksheet
Explore the principles of Mendelian inheritance with this monohybrid cross worksheet, covering genotypes, phenotypes, and probability.
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Monohybrid Cross Worksheet
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Read each question carefully and provide your answers in the space provided. Show all your work for monohybrid crosses, including Punnett squares where applicable.
1. An organism's genetic makeup is called its , while its observable characteristics are its .
2. An allele that is always expressed when present is , and an allele that is only expressed when two copies are present is .
3. A cross involving a single trait is known as a cross.
1. In pea plants, tall (T) is dominant over short (t). If a homozygous tall pea plant is crossed with a homozygous short pea plant, what will be the genotype and phenotype of the F1 generation?
2. Using the information from the previous question, if two F1 generation plants are crossed, what are the expected genotypic and phenotypic ratios of the F2 generation? Use the Punnett square below to help you.

1. If a heterozygous individual (Aa) is crossed with a homozygous recessive individual (aa), what percentage of the offspring are expected to be heterozygous?
0%
25%
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100%
1. A monohybrid cross always results in a 3:1 phenotypic ratio in the F2 generation.
True
False
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