Biology Lab Techniques Worksheet
This worksheet covers fundamental biology lab techniques including microscopy, sterile technique, and spectrophotometry, suitable for Grade 12 students.
Includes
Standards
Topics
Biology Lab Techniques
Name:
Date:
Score:
Read each question carefully and provide your best answer. Some questions may require critical thinking and application of biological principles.
1. Which of the following is the most appropriate technique to ensure sterility when transferring bacterial cultures?
Working near an open flame
Using sterile gloves and wiping down the bench with ethanol
Leaving petri dishes open for minimal time
All of the above
2. When using a compound microscope, which objective lens should you start with for initial focusing?
Scanning (4x)
Low power (10x)
High power (40x)
Oil immersion (100x)
1. A spectrophotometer measures the amount of light absorbed or transmitted by a sample.
True
False
2. The total magnification of a microscope is calculated by adding the ocular lens magnification to the objective lens magnification.
True
False
1. The process of separating cellular components by spinning them at high speeds is called .
2. When preparing a bacterial smear for Gram staining, the slide must be to fix the cells.
1. Describe two safety precautions that should always be followed when working with hazardous chemicals in a biology lab.
2. Explain the purpose of a blank in spectrophotometry.
Identify the labeled parts of the microscope below.
1.
2.
3.
4.
5.
6.
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